When an error occurs, the detector displays a specific message:
Loosen the thumb screws on the front panel cover to expose the lamp housing.
Unplug the lamp connector cable. Loosen the two fixing screws holding the lamp assembly in place and gently pull the lamp straight out.
Cycles through operational screens (Wavelength, Absorbance, Lamp Status). shimadzu spd20a user manual
The Shimadzu SPD-20A User Manual is an essential, structured resource that empowers users to achieve low-noise, stable UV/Vis detection in routine HPLC analysis. Following its guidelines ensures maximum lamp life, minimal downtime, and reproducible quantification from method development to quality control.
The SPD-20A can be operated directly via its front-panel keypad or integrated into chromatography data systems (CDS) like Shimadzu LabSolutions, LCsolution, or Agilent OpenLab. Step-by-Step Startup Procedure
The detector uses a holographic grating with 1600 grooves/mm to separate light into a spectrum. A key feature is the mirror M1, which physically selects the light source (D2 or W lamp). This switching is controlled by the operator via the "LAMP" setting on the keypad. For the SPD-20AV, filters are automatically inserted to cut higher-order spectra and stray light based on the selected wavelength range. When an error occurs, the detector displays a
Loosen the thumb screws on the right side panel of the detector and slide the cover off.
Flush the flow cell with a miscible, non-buffered solvent (e.g., HPLC-grade water followed by Methanol or Acetonitrile) to prevent salt precipitation or microbial growth.
: When the deuterium (D2) lamp is on, "D2" is shown on the detector's display. You can activate the lamp through the software method or the front panel keypad. Method Parameters : Wavelengths and other settings can be edited in the panel within your chromatography software. The SPD-20A can be operated directly via its
The Shimadzu SPD-20A is a high-performance UV-Vis detector used widely in High-Performance Liquid Chromatography (HPLC). It offers exceptional sensitivity, low noise levels, and dual-wavelength monitoring. This comprehensive guide serves as an operational manual, troubleshooting resource, and maintenance reference for laboratory technicians and researchers. 1. Specifications and Technical Overview
The is not a dusty binder to store on a shelf. It is an active diagnostic tool, a preventive maintenance schedule, and an educational resource. By familiarizing yourself with its structure—from the safety warnings in Chapter 1 to the error codes in the Appendix—you will reduce downtime, extend lamp life, and produce more reliable chromatographic data.